HER2 Equivocal Results - Genpathdiagnostics.com
Since implementing this strategic approach to HER2 FISH results in early 2016, our HER2 equivocal/borderline rate has been reduced from ~10% to an average of 3%. ... Read Content
Revised ASCO-CAP HER2 Test Guideline Recommendations
Must have a HER2 test performed in a metastatic site. HER2 may be tested with either immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH). If either test is equivocal, reflex testing using a different method or a new specimen must be performed. signals/cell (Negative: HER2 testing by IHC ... Fetch Document
Chromosome 17 Polysomy: Correlation With Histological ...
Correlation of Chromosome 17 Polysomy with HER2 IHC and Gene Amplification by FISH (N=266). Table 3. Summary of statistical findings. Figure 1. A biopsy from an ulcerated breast lesion revealed a high grade infiltrating ductal carcinoma (A). Her2 immunohisto-chemistry demonstrated 2+, equivocal staining (B). On FISH, more than two thirds ... Fetch Here
HER2 - Association For Molecular Pathology
HER2. expression did not alter sensitivity of the RT-PCR assay. Conclusions: q. RT-PCR analysis of . HER2. gene expression represents a viable approach to resolve cases with double equivocal . HER2. status at the time of diagnostic biopsy. This molecular approach accurately determined . HER2. status in a population that failed classification by ... Access Full Source
Laboratory Testing For Her2 Status In Breast Cancer
Laboratory Testing for Her2 Status in Breast Cancer May 28, 2015 Assistant Professor, Department of Pathology . Katherine Geiersbach, M.D. Overview • Clinical relevance of Her2 status for treatment of Resolution of Equivocal Her2 FISH ... Fetch Content
FISH For HER2 Gene Amplification - Cleveland Clinic Laboratories
FISH also may be useful as a confirmatory reflex assay for breast carcinoma cases for which an equivocal score has been identified previously by immunohistochemistry. Interpretation HER2 gene amplification is reported as either amplified, not amplified, or equivocal. The HER2 gene copy number is specified, and the HER2/chromosome 17 ratio is ... Content Retrieval
Management Of HER2 Equivocal Breast Cancer
• Retrospective review of all HER2 equivocal core biopsies diagnosed and treated at Levine Cancer Institute from 2014 through 2017. • HER2 equivocal specimens were defined using standards set forth by the 2013 ASCO/CAP guidelines: IHC 2+, single-probe FISH average HER2 copy number ≥4.0 and <6.0 signals/cell, or dual- ... Get Content Here
Use And Interpretation Of HER2 (ERBB2) IHC And FISH Methods
Prior to reporting an equivocal FISH result, additional cells are counted by a second observer and, if the result is still equiv ocal, the FISH assay is automatically repeated. This figure was developed by Quest Diagnostics based on reference 1. ... Retrieve Content
Automated Quantitative RNA In Situ Hybridization For ...
Negative, and 27 remained equivocal (ie, double equivocal)]. Evaluable HER2 mRNA was obtained for 154 cases (94%). Of the 154 cases, 31 with unequivocal HER2 status by FISH and IHC were pre-allocated into a training set and 74 with unequivocal HER2 status by FISH and IHC were pre-allocated into a validation set. The remaining 49 cases not ... View Full Source
Considered amplified or equivocal. This study examines the frequency and clinico-pathologic characteristics of breast cancers with equivocal or ‘non-classical’ HER2 FISH results. Breast cancers (2001–2014) with HER2 FISH results, HER2 immunohistochemistry, ER, grade, and age from three institutions (Stanford, UCSF, UWMC) were collected. ... Get Document
ASCO–CAP HER2 Test Guideline Recommendations
C,eDual-probe HER2/CEP17 ratio < 2.0; with an average HER2 copy number ≥6.0 signals/cell Equivocal for HER2 is defined as: IHC 2+ or FISH HER2/CEP17 ratio of 1.8-2.2 or average HER2 gene copy number 4-6 HER2 signals/nucleus for test systems without an internal control probe Must report HER2 test result as equivocal and order reflex test ... Fetch Full Source
Double-Equivocal HER2 Invasive Breast Carcinomas
Second case was negative (score 0) on IHC but equivocal on FISH and was HER2 positive by the alternate probe (Table, case 10). FISH was performed on this case given that an outside biopsy was reported as HER2 equivocal. The unresolved case was an IHC-negative (score 1þ)/FISH-equivocal case that remained equivocal by the alternate probe (Table ... Fetch Here
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